Molecular and Cellular Immunology Core and Becton-Dickinson Biosciences Presentation

Becton-Dickinson (BD), a company that makes flow cytometers, in collaboration with Dr. Alex Gurary (a flow core director at JABSOM), will hold a seminar on sorting with flow cytometers on Thursday, June 6, 2019, HIG 131 (SOEST video wall room), 10:00 a.m.

Dr. Karen Selph, Associate Specialist, UHM Dept. of Oceanography, will attend to help with any questions specific to the types of samples SOEST faculty and staff work with–as opposed to medical-field related samples, although Dr. Lisa Bellemare, who is with BD, also has experience in this area.

During this seminar, the basics of flow cytometry and cell sorting will be covered, including sample preparation for a variety types of samples–plankton, bacteria, yeasts, eukaryotic and prokaryotic cells–as well as instrument settings and the necessary correct controls.

Discussion will include the type of downstream analysis possible after cell sorting and the advantages of index sorting, which is the ability to sort single cells into 96 well/pcr plates and record the fluorescence levels of the one sorted cell, and tie that data with any downstream application. The capabilities and possibilities of such a powerful tool in single cell research will also be discussed. Light refreshments will be provided. For more information contact Alexandra Gurary at gurary@hawaii.edu or 692-1794.