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Seminar: Modular On-Site eDNA Enrichment (eDNA MODE): An Autonomous Sampling System for Active Environments
Austin Fisher, Walid Hassan, Olivia Holbrook, William Redford
ORE653: Ocean Instrumentation and Technology
*In-person ONLY (POST 723)*
*request Zoom Link here for extenuating circumstances by Friday (4/29) @ 11:59 pm HST
Environmental DNA (eDNA) samplers are in situ, autonomous water filtration systems that are designed to collect genetic material within the water column, enabling species detection in the lab. While there are over 20 eDNA samplers currently available, none offer a combination of high filtration rate, robust housing, self-preserving technology, and the capacity to collect replicate samples. We will present the design of the eDNA MODE (Modular On-site DNA Enrichment), an open-source eDNA sampler for deployment in high energy environments that aims to address these limitations. eDNA MODE leverages low-cost, off-the-shelf components to enable the filtration of 1 L triplicates in under 6 minutes (i.e., 1 L per 2 minutes) followed by immediate on-board preservation. In collaboration with Pacific Northwest National Laboratory (PNNL), we hope to deploy eDNA MODE at Sequim Bay, where tidal flows reach a peak current speed of up to 2 m/s in a 12 m deep channel, to assess the impacts of marine renewable energy on marine ecology.