Assistant Researcher

Hawaii Institute of Marine Biology

University of Hawaii

PO Box 1346

Kaneohe, HI  96744

for deliveries use street address 46-007 Lilipuna Road

Office:  (808) 236-7464

Lab:  (808) 236-7465

Fax:  (808) 236-7443

Email:  rappe@hawaii.edu

Education

1. •Ph.D., Genetics Program and Department of Microbiology, Oregon State University.  Advisor:  Dr. Stephen Giovannoni.  1997.
   

2. •B.S., Biology and University Honors Program, Washington State University.  1991.
   

Professional Experience

1. •Assistant Researcher, Hawaii Institute of Marine Biology, School of Ocean and Earth Science and Technology, University of Hawaii at Manoa.  2003-present.
   

2. •Postdoctoral Research Associate, Department of Microbiology, Oregon State University.  2000-2003.
   

3. •Postdoctoral Research Associate, College of Veterinary Medicine, Oregon State University.  1999-2000.
   

4. •Postdoctoral Research Associate, Phytoplankton Group, Station Biologique de Roscoff, France.  1997-1999.
   

5. •Co-organizer, Agouron Summer Course in Microbial Oceanography, Agouron Institute, the University of Hawaii, and NSF.  2006-present.
   

Professional Activities and Service

1. •Professional affiliations:  American Society for Microbiology, American Society of Limnology and Oceanography, International Society for Microbial Ecology, American Association for the Advancement of Science
   

2. •Peer reviewer:  Science, Nature, Microbiology and Molecular Biology Reviews, Proceedings of the National Academy of Science U.S.A., Applied and Environmental Microbiology, Environmental Microbiology, Limnology and Oceanography, Limnology and Oceanography Methods, FEMS Microbiology Ecology, ISME Journal, Aquatic Microbial Ecology, Proceedings of the Royal Society, London: Biological Sciences, BioTechniques, Ocean Science, Trends in Microbiology, Deep Sea Research II, Journal of Geophysical Research-Biogeosciences, NSF, NASA, NERC, NOAA, Hawaii SeaGrant.
   

3. •Graduate Faculty status, Departments of Oceanography and Microbiology, University of Hawaii Manoa.
   

Graduate Student Committees (*Advisor)

Marcie Workman, M.S., UH Oceanography, 2004-2005

Marina Brandon*, M.S., UH Oceanography, 2004-2006

Chris Ostrander, M.S., UH Oceanography, 2005-2007

Sara Yeo*, M.S., UH Oceanography, 2005-present

Amy Apprill*, Ph.D., UH Oceanography, 2005-present

Jennifer Salerno*, Ph.D., UH Zoology, 2005-present

Darin Hayakawa*, Ph.D., UH Microbiology, 2005-present

Tracy Campbell*, M.S., UH Oceanography, 2006-present

Olivia Mason, Ph.D., OSU Oceanography, 2006-2008

Jennifer Brum, Ph.D., UH Oceanography, 2007-present

Greg Concepcion, Ph.D., UH Zoology, 2008-present

Sean Jungbluth*, M.S., UH Oceanography, 2008-present

Undergraduate Student Research Supervision

Lei Young, Oregon State University, 2002-03

Friederike Baumann, Anhalt University, Germany, 2003-04

Cornelia Schmidt, Anhalt University, Germany, 2003-04

Sara Yeo, Hawaii Pacific University, 2004-05

Tracy Campbell, University Wisconsin Stout, 2005

Elizabeth Hambleton, Williams College, 2005

Tamara Molina, University of Hawaii, 2006

Aurora MacRae-Crerar, Barnard College, 2006

Jon Coloma, University of Hawaii, 2006-07

Chelsea Dudoit, University of Hawaii, 2006-08

Publications (links to a separate page)

Presentations and Abstracts

Apprill, A. M., H. Marlow, M. Martindale, and M. S. Rappé. Microbial associations during development of the coral Pocillopora meandrina. Talk presented at the 12th International Symposium on Microbial Ecology, Cairns, Australia, 17 - 22 August 2008.

Eiler, A., D. H. Hayakawa, M. J. Church, D. Karl, and M. S. Rappé.  Population structure and spatiotemporal distribution of the SAR11 clade in the subtropical North Pacific.  Talk presented at the 12th International Symposium on Microbial Ecology, Cairns, Australia, 17 - 22 August 2008.

Salerno, J., and M. S. Rappé.  Biogeography of bacterial communities associated with reef water and corals of the Hawaiian Archipelago and Greater Pacific Basin.  Poster presented at the 12th International Symposium on Microbial Ecology, Cairns, Australia, 17-22 August 2008.

Huggett, M. J., D. H. Hayakawa, S. J. Giovannoni, H. J. Tripp, and M. S. Rappé.  Genome streamlining and physiology of a widespread clade of coastal ocean methylotrophs - why carry a handbag when all you need is a wallet?  Poster presented at the 12th International Symposium on Microbial Ecology, Cairns, Australia, 17-22 August 2008.

Salerno, J., and M. S. Rappé.  Assessing the biogeographical distribution of bacterial communities associated with reef water and corals of the Papahānaumokuākea Marine National Monument and greater Pacific Basin.  Talk presented at the 2008 Hawaii Conservation Conference, Honolulu, Hawaii, USA, 29-31 July 2008.

Salerno, J., and M. S. Rappé.  Biogeography of reef water and coral-associated bacterial communities across the Hawaiian Archipelago and Greater Pacific Basin.  Talk presented at the 11th International Coral Reef Symposium, Fort Lauderdale, Florida, USA, 7-11 July 2008.

Apprill, A. M., H. Marlow, M. Martindale, and M. S. Rappé.  The onset of microbial associations in the developing coral Pocillopora meandrina. Poster presented at the 108th General Meeting of the American Society for Microbiology, Boston, Massachusetts, USA, 1-5 June 2008.

Apprill A. M., and M. S. Rappé. Water quality and microbial dynamics in reef waters following nutrient loading due to coral spawning events. Ocean Sciences Meeting, Orlando, Florida, USA, March 2-7, 2008.

Hayakawa, D. H., S. J. Giovannoni, U. Stingl, H. J. Tripp, S. Givan, J.-C. Cho, H.-M. Oh, J. Kitner, K. L. Vergin and M. S. Rappé.  The small genome of an abundant coastal ocean methylotroph.  Poster presented at the Genomes, Medicine and the Environment 2007 Conference, San Diego, California, USA, 8-10 October 2007.

Apprill, A. M., T. A. Mooney, A. K. Stimpert, M. O. Lammers, and M. S. Rappé.  A novel approach to examine environmental and health-related characteristics of Humpback Whales using the bacterial composition of epithelial skin.  Talk to be presented at the 17th Biennial Conference on the Biology of Marine Mammals, Cape Town, South Africa, 29 November - 3 December 2007.

Vergin, K. L., H. J. Tripp, L. J. Wilhelm, D. R. Denver, M. S. Rappé, and S. J. Giovannoni.  High intraspecific recombination in a native population of candidatus Pelagibacter ubique (SAR11).  Poster presented at the 107th General Meeting of the American Society for Microbiology, Toronto, Ontario, Canada, 21-25 May, 2007.

Van Mooy, B., S. Dyhrman, H. Fredricks, B. Pedler, M. Koblížek, L. Moore, T. Moutin, M. Brandon, M. Rappé, G. Rocap, and E. Webb.  Membrane lipid substitutions are a widely distributed and quantitatively significant biochemical mechanism for phytoplankton to alter nutrient requirements.  Talk presented at the 2007 ASLO Aquatic Sciences Meeting, Santa Fe, New Mexico, USA, 4-9 February 2007.

Rappé, M. S.  Coral-associated microorganisms in Kaneohe Bay and Northwestern Hawaiian Islands reef environments.  Invited seminar presented to the Department of Zoology, University of Hawaii at Manoa, Honolulu, Hawaii, USA, 13 October 2006.

Cowen, James P., B. T. Glazer, M. Rappé, A. Fisher, K. Becker, E. Davis, H. Jannasch, and G. Wheat.  Microbial geochemical and tracer transport studies of ocean basement using ODP CORK Observatories.  Poster presented at the EGU General Assembly, Vienna, Austria, 2-7 April 2006.

Becker, J. W., and M. S. Rappé.  Mesoscale to microscale: molecular microbial ecology within cyclonic eddies in the lee of the Hawaiian Islands.  Poster presented at the 2006 Ocean Sciences Meeting, Honolulu, Hawaii, USA, 20-24 February 2006.

Hayakawa, D. H, and M. S. Rappé.  Isolation and molecular ecology of novel Beta Proteobacteria from sub-tropical coastal seawater.  Talk presented at the 2006 Ocean Sciences Meeting, Honolulu, Hawaii, USA, 20-24 February 2006.

Brandon, M. L., and M. S. Rappé.  High-throughput isolation of marine bacterioplankton from Kane`ohe Bay, Hawaii.  Talk presented at the 2006 Ocean Sciences Meeting, Honolulu, Hawaii, USA, 20-24 February 2006.

Salerno, J. L., A. Apprill, E. Hambleton, and M. S. Rappé.  Assessing the composition of microbial communities associated with colonies of diverse coral species in the main and Northwestern Hawaiian Islands.  Talk presented at the 2006 Ocean Sciences Meeting, Honolulu, Hawaii, USA, 20-24 February 2006.

Glazer, B. T., J. P. Cowen, D. Copson, D. Harris, J. Jolly, D. B. Nuzzio, J. W. Becker and M. S. Rappé.  A seafloor instrument package for in situ geochemical measurements concurrent to in situ filtration.  Poster presented at the 2006 Ocean Sciences Meeting, Honolulu, Hawaii, USA, 20-24 February 2006.

Rappé, M. S.  Expanding the traditional cultivation-based repertoire to isolate bacterioplankton for new model biosystems in microbial oceanography.  Invited tutorial lecture presented at the 2005 ASLO Aquatic Sciences Meeting, Salt Lake City, Utah, USA, 20-25 February 2005.

Rappé, M. S.  Melding cultivation-based and molecular ecological approaches to isolate and study new model biosystems in microbial oceanography.  Invited seminar presented to the Department of Microbiology, Cornell University, Ithaca, New York, 27 January 2005.

Rappé, M. S.  An introduction to the ARB sequence analysis package.  Invited workshop presented at Cornell University, Ithaca, New York, 26 January 2005.

Zengler, K., G. Clark, I. Haller, G. Toledo, M. Walcher, M. Rappé, G. Woodnut, J. M. Short, M. Keller.  Accessing microbial diversity by high throughput cultivation.  Talk presented at the 10th International Symposium on Microbial Ecology, Cancun, Mexico, 22-27 August 2004.

Rappé, M. S.  Marine microbial ecology: Linking global processes to microbial community structure and genomes.  Invited talk presented at the Marine Biotechnology Outreach and Education Project Curriculum Development Workshop, Oregon and Hawaii SeaGrant, Hawaii Institute of Marine Biology, Kaneohe, Hawaii, USA, 28 July 2004.

Morris, R. M., M. S. Rappé, J. C. Cho, K. Vergin, C. A. Carlson, and S. J. Giovannoni.  Seasonal shifts in bacterioplankton community structure at BATS.  Poster presented at the 104th General Meeting of the American Society for Microbiology, New Orleans, Louisiana, USA, 23-27 May 2004.

Rappé, M. S.  Using over 10 years of rRNA data to guide a new generation of cultivation- and genomics-based research in marine microbial ecology.  Invited talk presented at the Department of Zoology, University of Hawaii at Manoa, Honolulu, Hawaii, USA, 30 April 2004.

Morris, R. M., M. S. Rappé, K. L. Vergin, J. Oliver, R. Parsons, C. A. Carlson, and S. J. Giovannoni.  In situ and experimental dynamics of group specific bacterioplankton: recent highlights from an oceanic microbial observatory.  Poster presented at the Second Microbial Observatories Principal Investigators' Workshop, Washington DC, USA, 14-16 September 2003.

Morris, R. M., T. D. Wright, E. Urbach, M. S. Rappé, K. L. Vergin, S. A. Connon, and S. J. Giovannoni.  Direct cell counts reveal mesopelagic distribution of the Green Non-Sulfur (GNS) related SAR202 bacterioplankton group.  Talk presented at the 2003 ASLO Aquatic Sciences Meeting, Salt Lake City, Utah, USA, 9-14 February 2003.

Rappé, M. S.  The SAR11 marine bacterioplankton group:  cultivation of one of the most abundant organisms on Earth.  Invited talk presented at the University of Hawaii, School of Ocean and Earth Science and Technology, Honolulu, Hawaii, USA, 24 June 2002.

Rappé, M. S., and S. J. Giovannoni.  The major marine bacterioplankton rRNA groups:  diversity, distribution, dynamics and cultivation.  Invited talk presented at the 2002 ASLO Summer Meeting, Victoria, B.C., 10-14 June 2002.

Rappé, M. S., R. M. Morris, K. L. Vergin, and S. J. Giovannoni.  Pelagibacter ubique gen. nov., sp. nov., characterization of the first isolates from the SAR11 marine bacterioplankton clade from the Pacific Ocean.  Poster presented at the 102nd General Meeting of the American Society for Microbiology, Salt Lake City, Utah, USA, 17-21 May 2002.

Alexander, C. A., M. S. Rappé, K. L. Vergin, and S. J. Giovannoni.  Identification of marine bacteria associated with phytoplankton blooms in Oregon coastal waters.  Poster presented at the 102nd General Meeting of the American Society for Microbiology, Salt Lake City, Utah, USA, 17-21 May 2002.

Rappé, M. S., S. A. Connon, K. L. Vergin, and S. J. Giovannoni.  Cultivation of the ubiquitous marine bacterium SAR11.  Talk presented at the 2002 Ocean Sciences Meeting, Honolulu, Hawaii, USA, 11-15 February 2002.

Morris, R. M., S. A. Connon, M. S. Rappé, K. L. Vergin, W. A. Siebold, C. Carlson, and S. J. Giovannoni.  In-situ abundance of the SAR11 bacterioplankton clade in the North Atlantic Ocean.  Talk presented at the 2002 Ocean Sciences Meeting, Honolulu, Hawaii, USA, 11-15 February 2002.

Giovannoni, S., and M. S. Rappé.  Patterns in the distributions of the marine bacterial groups in the oceans.  Talk presented at the 9th International Symposium on Microbial Ecology, Amsterdam, The Netherlands, 26-31 August 2001.

Smith, E. A., J. Macrae, F. Grant, S. Gallacher, G. L. Hold, and M. S. Rappé.  Characterization and phylogenetic diversity of bacteria associated with toxic and non-toxic algae.  Talk presented at the 7th European Marine Microbiology Symposium, Noordwijkerhout, The Netherlands, 17-22 September 2000.

Brinkmeyer, R., M. Rappé, K. Töbe, D. Vaulot, S. Gallacher, and L. K. Medlin.  Development of clade (Roseobacter and Alteromonas) and species-specific oligonucleotide probes to study bacterial/algal interactions and their role in Harmful Algal Bloom ecology.  Poster presented at the 2nd European Phycological Conference, Montecatini Terme, Italy, 20-26 September 1999.

Smith, E. A., G. L. Hold, M. Rappé, C. M. J. Ferguson, F. Milne, and S. Gallacher.  Enumeration and phylogenetic analysis of bacteria associated with toxic and non-toxic dinoflagellates:  Alexandrium spp. and Scrippsiella trochoidea.  Talk presented at the Aquatic Associations of the UK Conference “Microbial Aquatic Symbiosis:  From Phylogeny to Biotechnology,” Oban, Scotland, 1-3 September 1999.

Giovannoni, S. J., M. Rappé, and B. Lanoil. What we have learned from a decade of studying picoplankton diversity using molecular techniques, and the application of this knowledge to the identification of single cells.  Talk presented at the 1999 ASLO Aquatic Sciences Meeting, Santa Fe, New Mexico, USA, 1-5 February 1999.

Rappé, M. S., G. Hold, S. Gallacher, R. Brinkmeyer, M. Lange, L. K. Medlin, and D. Vaulot.  Diversity and population dynamics of bacteria in co-culture with the marine dinoflagellates Scrippsiella trochoidea and Alexandrium tamarense.  Poster presented at the 98th General Meeting of the American Society for Microbiology, Atlanta, Georgia, USA, 17-21 May 1998.

Rappé, M. S., K. Vergin, and S. J. Giovannoni.  Diversity of plastid-derived 16S rRNA genes cloned from bulk environmental DNA.  Poster presented at the 1st European Phycological Congress, Cologne, Germany, 11-18 August 1996.

Giovannoni, S. J., and M. S. Rappé.  Molecular ecological approaches for assessing phytoplankton diversity and population dynamics.  Talk presented at the 1st European Phycological Congress, Cologne, Germany, 11-18 August 1996.

Suzuki, M. T., Z. W. Haimberger, M. S. Rappé, H. Winfield, J. Ströbel, and S.J. Giovannoni.  A comparison between the 16S rRNA of marine bacterioplankton strains isolated from the Oregon coast to those cloned from bulk DNA from the same water sample.  Talk presented at the 7th International Symposium on Microbial Ecology, Sao Paulo, Brazil, 27 August - 1 September 1995.

Giovannoni, S. J., M. T. Suzuki, and M. S. Rappé.  Bacterioplankton diversity:  uncultured or unculturable?  Talk presented at the 7th International Symposium on Microbial Ecology, Sao Paulo, Brazil, 27 August - 1 September 1995.

Rappé, M. S., and S. J. Giovannoni.  Analysis of marine coastal picoplankton 16S rRNA genes cloned from the continental shelf at Cape Hatteras.  Poster presented at the 95th General Meeting of the American Society for Microbiology, Washington DC, USA, 21-25 May 1995.

Rappé, M. S., P. F. Kemp, and S. J. Giovannoni.  Abundant chromophyte plastid 16S ribosomal RNA genes found in a clone library from Atlantic Ocean seawater.  Poster presented at the 9th Northwest Algal Symposium, Anacortes, Washington, USA, 31 March - 2 April 1995.

Rappé, M. S., P. F. Kemp, and S. J. Giovannoni.  Phylogenetic analysis of coastal marine bacterioplankton by 16S rRNA gene cloning from a natural plankton population.  Talk presented at the 1994 Ocean Sciences Meeting, San Diego, California, USA, 21-25 February 1994.

Rappé, M. S., N. L. Fowles, and S. J. Giovannoni.  Identification of a novel microbial 16S rRNA lineage of the Chloroflexus/Herpetosiphon phylum in Sargasso Sea bacterioplankton.  Poster presented at the Northwest Branch Meeting of the American Society for Microbiology, Pullman, Washington, USA, 17-19 June 1993.

Rappé, M. S., N. L. Fowles, and S. J. Giovannoni.  Identification of a novel microbial 16S rRNA lineage of the Chloroflexus/Herpetosiphon phylum in Sargasso Sea bacterioplankton.  Poster presented at the ASLO, SWS Annual Meeting, Edmonton, Alberta, Canada, 30 May - 3 June 1993.