Oceanography 633:Biogeochemical Methods in Oceanography
Fall
2007
Teaching Assistant:
Tina Lin
Guest Instructors:
Drs. James P. Cowen, Jane Schoonmaker, Karen Selph, Guang Yi
Wang
Note: Because labs tend to require more than the three
hours scheduled each week and because of faculty conflicting schedules, the
course will begin Monday August 27,
the SECOND WEEK of classes. Theory and
the basis of methods will be presented on Mondays and Wednesdays and laboratory
exercises and other equipment demonstrations will take place during the Friday
extended laboratory sessions.
The field trip to Kaneohe
Bay will require at least a half-day and may cause students to miss other
classes. We will attempt to schedule
the field trip after consultation with students so as to minimize interference
with other commitments. It is also possible that we will run the field trip on a
weekend, although the availability of a boat/pilot from HIMB will dictate
whether this is possible or not. The day of the field trip will be long because
some samples must be processed immediately after collection. Other samples may
need to be processed within a few days. No lab will be held the week following
the field trip to compensate for the extra time spent with sample processing,
|
Date |
|
Topic |
Instructor(s) |
|
August |
27 |
Course Introduction
|
CIM, FJS |
|
|
29 |
Quantification,
detection limits, blanks, sources of error, significant figures, data
analysis |
CIM |
|
|
31 |
Good Laboratory
Practices, lab ware, weighing, pipetting, calibration of volumetric lab
ware |
TL, CIM |
|
|
|
|
|
|
September |
3 |
Holiday: Labor
Day
|
|
|
|
5 |
Water samplers,
Sediment samplers |
FJS |
|
|
7 |
Navigation,
GPS |
TL, FJS |
|
|
|
|
|
|
September |
10 |
Environmental
monitoring equipment. multiparameter probes, other in-situ
systems |
EDC |
|
|
12 |
Environmental monitoring systems
(cont.) |
EDC |
|
|
14 |
Field Trip (and
sample processing) |
TL, EDC, FJS |
|
|
|
|
|
|
September |
17 |
Conductivity, Salinity,
T-S diagrams |
CIM |
|
|
19 |
CTD,
Minisal/AutoSal |
CIM |
|
|
21 |
LAB
HOLIDAY |
|
|
|
|
|
|
|
September |
24 |
Theory of
titrimetry |
EDC |
|
|
26 |
Applications of
titrimetry (CL, Ca, Mg, Alk) |
EDC |
|
|
28 |
Analysis of CL, Ca, Mg
in seawater |
TL, EDC |
|
|
|
|
|
|
October |
1 |
Acid/Base
theory |
EDC |
|
|
3 |
Dissolved inorganic
carbon, alkalinity |
EDC |
|
|
5 |
Alkalinity
titrations |
TL, EDC, R Solomon |
|
|
|
|
|
|
October |
8 |
Flow Injection Analysis theory
CLASS at 1:30 this week |
CIM, W. Hiscock J. Ruzicka |
|
|
10 |
FIA systems (ICP-MS,
Fluorescence) |
CIM, W. Hiscock J. Ruzicka |
|
|
12 |
Fe, Al in seawater with
FIA/fluorescence detection |
TL, CIM, W Hiscock |
|
|
|
|
|
|
October |
15 |
Spectrometric methods
of analysis, theory |
EDC |
|
|
17 |
ICP-AES and
ICP-MS |
EDC |
|
|
19 |
ICP-MS analysis of Mn
in porewater (or analysis of Ba, Rb in seawater) |
TL, EDC |
|
|
|
|
|
|
October |
22 |
Chromatography, dissolved methane
|
FJS |
|
|
24 |
Dissolved gases:
Winkler titration, oxygen meter |
FJS |
|
|
26 |
Dissolved gases: Winkler titration, oxygen
meter |
TL, FJS |
|
|
|
|
|
|
October |
29 |
UV visible spectrometry |
EDC, |
|
November |
1 |
UV-Vis application to
nutrient analysis |
EDC |
|
|
3 |
Inorganic
nutrients: spectrophotometry,
AutoAnalyzer, APNA |
TL, EDC, K. Fogaren P.
Bienfang |
|
|
|
|
|
|
November
|
5 |
X-ray Diffractometry
(Theory) |
EDC |
|
|
7 |
X-ray Diffractometry
(Demo) |
EDC, JS |
|
|
9 |
Demo of SEM
laboratory |
TL, J. Cowen, EDC |
|
|
|
|
|
|
November |
12 |
Holiday – Veterans’ Day |
|
|
|
14 |
Flow cytometry/Coulter
Counters |
K. Selph |
|
|
16 |
Flow
cytometry/Coulter Counters, demonstration |
TL, K. Selph |
|
|
|
|
|
|
November |
19 |
Detection of Bacteria in Sediments Polymerase Chain
Reaction (PCR) |
GY Wang |
|
|
21 |
Detection of
Bacteria/ PCR |
GY Wang |
|
|
23 |
Holiday –
Thanksgiving
|
|
|
|
|
|
|
|
November |
26 |
Detection of Bacteria/ PCR) |
GY Wang |
|
|
28 |
Detection of Bacteria/(PCR |
GY Wang |
|
|
30 |
DNA extraction, PCR |
TL, GY Wang |
|
|
|
|
|
|
December |
3 |
Student
Presentations |
EDC, CIM, FS |
|
|
5 |
Student
Presenations |
EDC, CIM,
FS |