Price List

Price List

Biogeochemical Stable Isotope Facility

School of Ocean and Earth Science and Technology

* Prices vary with the type of sample analyzed. Some sample materials with highly variable content of individual amino acids can require additional analysis. Please inquire directly for more information.

** Additional charges for sample preparation

*** Please see details on description page

 

Also looking for nutrient analysis? We can work closely with S-LAB to facilitate combined sample submission. Please visit the website for S-LAB at:

http://www.soest.hawaii.edu/S-LAB/

 

* The per sample cost for compound specific isotope analysis of amino acids performed at or by the SOEST Biogeochemical Stable Isotope Facility yields isotopic measurement (δ15N and/or δ13C) of 14 amino acids and includes rigorous quality control and assurance measures.

 

Amino acids are chemically isolated and purified from sample material prior to derivitization and esterification in preparation for GC-IRMS analysis.  Samples are then analyzed for δ15N using either a Thermo Scientific Delta V Plus or a Thermo Scientific MAT 253 mass spectrometer interfaced with a Thermo Finnigan Trace GC gas chromatograph via a Thermo Finnigan GC-C III. For δ13C, samples are analyzed using a Thermo Scientific Trace GC Ultra gas chromatograph via a Thermo Scientific Conflo IV.

 

Amino acids measured using this technique include alanine (Ala), glycine (Gly), threonine (Thr), serine (Ser), valine (Val), leucine (Leu), isoleucine (Iso), proline (Pro), methionine (Met), phenylalanine (Phe), tyrosine (Tyr) and lysine (Lys). Additionally, the terminal amide groups in glutamine (Gln) and aspartamine (Asn) are cleaved during the chemical isolation of amino acids, the result being the conversion of these amino acids to glutamic acid (Glu) and aspartic acid (Asp), respectively. Thus, the isotope value of a combined Glu + Gln is measured (termed Glx), and the isotope value of a combined Asn + Asp is measured (termed Asx).

 

For δ15N, internal reference compounds, L-2-Aminoadipic acid (AAA) and L-(+)-Norleucine (Nor) of known nitrogen isotopic composition, are co-injected with samples and used to normalize the measured isotope values of unknown amino acids and determine accuracy and precision. Between triplicate runs of each sample, a suite of amino acids (Ala, Thr, Iso, Pro, Glu, and Phe) of known isotopic composition are analyzed; the AAA and Nor are also co-injected with these amino acids. For isotopic correction of unknown amino acids when co-injected standards are unusable due to co-elution effects, a linear correction is derived from the amino acid suites run immediately before and after the triplicate sample analysis and applied to measured isotope ratios. All amino acids are analyzed in triplicate and isotopic values are reported in δ-notation relative to atmospheric N2.

 

For δ13C, internal reference compounds, L-2-Aminoadipic acid (AAA), L-(+)-Norleucine (Nor) and un-derivitized n-C20 alkane, of known carbon isotopic composition, are co-injected with samples and used to determine accuracy and precision. Between triplicate runs of each sample, a suite of all 14 amino acids prepared with your samples (Ala, Gly, Thr, Ser, Val, Leu, Iso, Asp, Pro, Met, Glu, Phe, Tyr and Lys), and of known isotopic composition, are analyzed; the AAA, Nor and n-C20 are also co-injected with these amino acids. For isotopic correction of unknown amino acids, an average correction factor is derived from the amino acid suites run before and after the triplicate sample analysis and applied to measured isotope ratios. All amino acids are analyzed in triplicate and isotopic values are reported in δ-notation relative to V-PDB.

 

 

Analysis

Price ($)

 

 

Gas Bench Sample Analysis

 

δ15N and δ18O Nitrate as N2O, by user

25.00

δ15N and δ18O Nitrate as N2O, by lab

45.00

Dissolved Inorganic Carbon

18.00

 

 

GC-combustion-mass spectrometer analysis

 

δ15N of Amino Acids, by user

195.00*

δ13C of Amino Acids, by user

205.00*

δ15N of Amino Acids, by lab

395.00*

δ13C of Amino Acids, by lab

405.00*

 

 

Elemental Analyzer**

 

δ13C, δ15N for plant and animal tissue***

11.00/18.50***

δ13C, δ15N on GF/F filters

17.00

δ13C, δ15N for sediments

15.00

 

 

Picarro

 

δ18O and δD of waters

8.00